sv40 dna atcc Search Results


sv40 genomic dna  (ATCC)
93
ATCC sv40 genomic dna
Sv40 Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sv40+dna+atcc/SV40+purified+plasmid+DNA%3B+Strain+776/pmc04627959-90-0-3
Average 93 stars, based on 1 article reviews
sv40 genomic dna - by Bioz Stars, 2026-10
93/100 stars
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sv40  (ATCC)
86
ATCC sv40
FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for <t>SV40</t> large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).
Sv40, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sv40+dna+atcc/Molecular+Clone+of+SV40+DNA/pm16052520-70-4-12
Average 86 stars, based on 1 article reviews
sv40 - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier

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FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for SV40 large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).

Journal: International journal of cancer

Article Title: No evidence for simian virus 40 DNA sequences in malignant non-Hodgkin lymphomas.

doi: 10.1002/ijc.21346

Figure Lengend Snippet: FIGURE 1 – Standard curves for quanti- tative real-time PCR (TaqMan) assays for SV40 large T antigen, EBV-BKRF-1 (EBNA-1), human K-ras and t(14;18). Different linearized plasmid DNAs con- taining the cloned target DNA fragments or the complete SV40 viral genome were 2-fold serial diluted (105 copies to <1 copy) and 3 replicates were tested. Mean cycle threshold values (CT) of 3 replicate tests were plotted against the log copy number of each gene or the cloned viral DNA. The standard curves were obtained by linear regression analysis. In the case of the cloned 111 bp fragment of SV40 large T antigen the standard SV40 DNA dilutions were made with and without the addition of human genomic DNA isolated from PBMNC from healthy individuals to give a concentration of human genomic DNA of 1 lg/50 ll per assay. This did not lead to changes of the standard curves (one example is shown in this figure).

Article Snippet: In the case of SV40 a linearized cloned viral DNA (strain pUCS40-CPC/MEN-1 [ATCC catalog No. VRMC-4]) as well as a cloned amplification DNA fragment were used as standards for quantification.

Techniques: Real-time Polymerase Chain Reaction, Plasmid Preparation, Clone Assay, Isolation, Concentration Assay